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empty vector pbabe puro  (Addgene inc)


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    Structured Review

    Addgene inc empty vector pbabe puro
    Empty Vector Pbabe Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 581 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbabe+puro+empty+vector/pBABE-puro+(Plasmid+%231764)/pmc12741039-149-2-5
    Average 96 stars, based on 581 article reviews
    empty vector pbabe puro - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Retroviral:

    Article Title: Escape from oncogene-induced senescence is controlled by POU2F2 and memorized by chromatin scars
    Article Snippet: The number and size of colonies were counted manually using Zen Blue 2.3 software (Zeiss). .. The following retroviral plasmids were used: pBABE-puro H-RASG12V; Addgene plasmid 1768, pBABE-puro Empty Vector; Addgene plasmid 1764, 90 pBABE-Neo hTERT; Addgene plasmid 1774, 91 pLNCX2 ER: Ras; Addgene plasmid 67844. .. 92 All plasmids were produced in MAX EfficiencyTM DH5α Competent Cells (Thermo Fisher) and isolated with EndoFree Plasmid Maxi Kit (Qiagen) according to the manufacturer's instructions.

    Article Title: PML restrains p53 activity and cellular senescence in clear cell renal cell carcinoma
    Article Snippet: .. Retroviral particles were generated by transfecting Phoenix-AMPHO cells with pBabe-puro Empty vector (Addgene #1764) or pBabe-puro-p53DD (generated by O. Moshe) with calcium phosphate according to the de Lange laboratory protocol ( https://delangelab.org/protocols ). ..

    Article Title: Escape from oncogene-induced senescence is controlled by POU2F2 and memorized by chromatin scars
    Article Snippet: .. Retroviral transductions The following retroviral plasmids were used: pBABE-puro H-RASG12V; Addgene plasmid 1768, pBABE-puro Empty Vector; Addgene plasmid 1764, 90 pBABE-Neo hTERT; Addgene plasmid 1774, 91 pLNCX2 ER: Ras; Addgene plasmid 67844. .. 92 All plasmids were produced in MAX EfficiencyTM DH5α Competent Cells (Thermo Fisher) and isolated with EndoFree Plasmid Maxi Kit (Qiagen) according to the manufacturer's instructions.

    Article Title: Escape from oncogene-induced senescence is controlled by POU2F2 and memorized by chromatin scars.
    Article Snippet: .. The following retroviral plasmids were used: pBABE-puro H-RASG12V; Addgene plasmid 1768, pBABE-puro Empty Vector; Addgene plasmid 1764,90 pBABE-Neo hTERT; Addgene plasmid 1774,91 pLNCX2 ER: Ras; Addgene plasmid 67844.92 All plasmids were produced inMAX EfficiencyTM DH5aCompetent Cells (Thermo Fisher) and isolated with EndoFree PlasmidMaxi Kit (Qiagen) according to the manufacturer’s instructions. .. GM21-, WI38-, and LF1-H-RASG12V fibroblasts and their empty vector counterparts were generated by retroviral transduction, as described in.24 Briefly, using the calcium phosphate precipitation method, retroviral particles were produced by transfecting the packaging cell lines, Platinum-A (Cell Biolabs), with the vector of interest.

    Plasmid Preparation:

    Article Title: Escape from oncogene-induced senescence is controlled by POU2F2 and memorized by chromatin scars
    Article Snippet: The number and size of colonies were counted manually using Zen Blue 2.3 software (Zeiss). .. The following retroviral plasmids were used: pBABE-puro H-RASG12V; Addgene plasmid 1768, pBABE-puro Empty Vector; Addgene plasmid 1764, 90 pBABE-Neo hTERT; Addgene plasmid 1774, 91 pLNCX2 ER: Ras; Addgene plasmid 67844. .. 92 All plasmids were produced in MAX EfficiencyTM DH5α Competent Cells (Thermo Fisher) and isolated with EndoFree Plasmid Maxi Kit (Qiagen) according to the manufacturer's instructions.

    Article Title: Fn14 promotes myoblast fusion during regenerative myogenesis
    Article Snippet: .. A pBABE-Puro empty vector and a pBABE-Puro-EGFP plasmid were purchased from Addgene. ..

    Article Title: Escape from oncogene-induced senescence is controlled by POU2F2 and memorized by chromatin scars
    Article Snippet: .. Retroviral transductions The following retroviral plasmids were used: pBABE-puro H-RASG12V; Addgene plasmid 1768, pBABE-puro Empty Vector; Addgene plasmid 1764, 90 pBABE-Neo hTERT; Addgene plasmid 1774, 91 pLNCX2 ER: Ras; Addgene plasmid 67844. .. 92 All plasmids were produced in MAX EfficiencyTM DH5α Competent Cells (Thermo Fisher) and isolated with EndoFree Plasmid Maxi Kit (Qiagen) according to the manufacturer's instructions.

    Article Title: Escape from oncogene-induced senescence is controlled by POU2F2 and memorized by chromatin scars.
    Article Snippet: .. The following retroviral plasmids were used: pBABE-puro H-RASG12V; Addgene plasmid 1768, pBABE-puro Empty Vector; Addgene plasmid 1764,90 pBABE-Neo hTERT; Addgene plasmid 1774,91 pLNCX2 ER: Ras; Addgene plasmid 67844.92 All plasmids were produced inMAX EfficiencyTM DH5aCompetent Cells (Thermo Fisher) and isolated with EndoFree PlasmidMaxi Kit (Qiagen) according to the manufacturer’s instructions. .. GM21-, WI38-, and LF1-H-RASG12V fibroblasts and their empty vector counterparts were generated by retroviral transduction, as described in.24 Briefly, using the calcium phosphate precipitation method, retroviral particles were produced by transfecting the packaging cell lines, Platinum-A (Cell Biolabs), with the vector of interest.

    Article Title: Fibroblast growth factor–inducible 14 regulates satellite cell self-renewal and expansion during skeletal muscle repair
    Article Snippet: Cultures were transfected with control or STAT3 siRNA (Santa Cruz Biotechnology) using Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific). .. A pBABE-Puro empty vector and a pBABE-Puro-EGFP plasmid were purchased from Addgene. ..

    Generated:

    Article Title: PML restrains p53 activity and cellular senescence in clear cell renal cell carcinoma
    Article Snippet: .. Retroviral particles were generated by transfecting Phoenix-AMPHO cells with pBabe-puro Empty vector (Addgene #1764) or pBabe-puro-p53DD (generated by O. Moshe) with calcium phosphate according to the de Lange laboratory protocol ( https://delangelab.org/protocols ). ..

    Produced:

    Article Title: Escape from oncogene-induced senescence is controlled by POU2F2 and memorized by chromatin scars.
    Article Snippet: .. The following retroviral plasmids were used: pBABE-puro H-RASG12V; Addgene plasmid 1768, pBABE-puro Empty Vector; Addgene plasmid 1764,90 pBABE-Neo hTERT; Addgene plasmid 1774,91 pLNCX2 ER: Ras; Addgene plasmid 67844.92 All plasmids were produced inMAX EfficiencyTM DH5aCompetent Cells (Thermo Fisher) and isolated with EndoFree PlasmidMaxi Kit (Qiagen) according to the manufacturer’s instructions. .. GM21-, WI38-, and LF1-H-RASG12V fibroblasts and their empty vector counterparts were generated by retroviral transduction, as described in.24 Briefly, using the calcium phosphate precipitation method, retroviral particles were produced by transfecting the packaging cell lines, Platinum-A (Cell Biolabs), with the vector of interest.

    Isolation:

    Article Title: Escape from oncogene-induced senescence is controlled by POU2F2 and memorized by chromatin scars.
    Article Snippet: .. The following retroviral plasmids were used: pBABE-puro H-RASG12V; Addgene plasmid 1768, pBABE-puro Empty Vector; Addgene plasmid 1764,90 pBABE-Neo hTERT; Addgene plasmid 1774,91 pLNCX2 ER: Ras; Addgene plasmid 67844.92 All plasmids were produced inMAX EfficiencyTM DH5aCompetent Cells (Thermo Fisher) and isolated with EndoFree PlasmidMaxi Kit (Qiagen) according to the manufacturer’s instructions. .. GM21-, WI38-, and LF1-H-RASG12V fibroblasts and their empty vector counterparts were generated by retroviral transduction, as described in.24 Briefly, using the calcium phosphate precipitation method, retroviral particles were produced by transfecting the packaging cell lines, Platinum-A (Cell Biolabs), with the vector of interest.



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